| 產(chǎn)品編號 | bs-1114R |
| 英文名稱 | beta Adducin Rabbit pAb |
| 中文名稱 | 內(nèi)收蛋白抗體 |
| 別 名 | ADD 2; ADD B; ADD2; ADDB; ADDL; Adducin 2(beta); Adducin 2; Adducin like; Adducin like protein 70; Adducin2; beta adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; Erythrocyte adducin subunit beta; MGC3339; MGC44427; ADDB_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 結(jié)合蛋白 細胞類型標志物 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human beta Adducin protein: 401-500/725 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. Function: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit. Subunit: Heterodimer of an alpha and a beta subunit. Subcellular Location: Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Tissue Specificity: Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the aldolase class II family. Adducinbn subfamily. SWISS: P35612 Gene ID: 119 Database links: Entrez Gene: 119 Human Entrez Gene: 11519 Mouse Omim: 102681 Human SwissProt: P35612 Human SwissProt: Q9QYB8 Mouse Unigene: 188528 Human Unigene: 11353 Rat 內(nèi)收蛋白(Adducin）是一種膜骨架蛋白，內(nèi)收蛋白可與肌動蛋白及血影蛋白復(fù)合體結(jié)合，并且通過Ca2+和鈣調(diào)蛋白的作用影響骨架蛋白的穩(wěn)定性，從而影響紅細胞的形態(tài). |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with beta Adducin polyclonal antibody, unconjugated (bs-1114R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
