| 產品編號 | bs-1164R |
| 英文名稱 | Perilipin-2 Rabbit pAb |
| 中文名稱 | 脂肪分化相關蛋白抗體 |
| 別 名 | ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; PLIN2; PLIN2_HUMAN; adipophilin; Adipose differentiation related protein; ADRP; MGC10598 |
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Specific References (4) | bs-1164R has been referenced in 4 publications.
[IF=8.47] Amrutkar, Manoj, et al. "Genetic Disruption of Protein Kinase STK25 Ameliorates Metabolic Defects in a Diet-Induced Type 2 Diabetes Model."Diabetes (2015). Mouse.
[IF=7.097] Yilan Shen et al. VEGF-B antibody and interleukin-22 fusion protein ameliorates diabetic nephropathy through inhibiting lipid accumulation and inflammatory responses. IF ; mouse.
[IF=2.2] Liu et al. Oxidized low-density lipoprotein activates adipophilin through ERK1/2 signal pathway in RAW264.7 cells. (2010) Acta.Biochim.Biophys.Sin.(Shanghai. 42:635-45 WB ; Mouse.
[IF=1.851] Liu Q et al. Inhibitory effect of 17β?estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2. Molecular Medicine Reports.2019,19: 5087-5096 . Rat&Mouse.
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| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADFP: 31-130/422 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] Function: May be involved in development and maintenance of adipose tissue. Subcellular Location: Membrane; Peripheral membrane protein. Tissue Specificity: Milk lipid globules. Post-translational modifications: Acylated; primarily with C14, C16 and C18 fatty acids. Similarity: Belongs to the perilipin family. SWISS: Q99541 Gene ID: 123 Database links: Entrez Gene: 123 Human Entrez Gene: 11520 Mouse Omim: 103195 Human SwissProt: Q99541 Human SwissProt: P43883 Mouse Unigene: 3416 Human Unigene: 381 Mouse Adipophilin（ADFP）是一種脂滴周圍主要的相關蛋白，它大量分布在脂質蓄積正?；虿徽５募毎校侵|蓄積的一個特異性標記物。它在脂肪細胞分化早期就有很高的表達，但當脂肪細胞成熟后，它的表達就明顯減少，故稱；脂肪分化相關蛋白。 ADFP在很多組織器官中都發(fā)揮重要作用，它不僅參與脂肪細胞的脂質代謝、脂滴的形成及肝內三酰甘油（TO）的合成與代謝，還能促進巨噬細胞、平滑肌細胞的泡沫化，長鏈脂肪酸的攝取，乳汁的分泌等。 ADFP的表達異常與動脈粥樣硬化、胰島素抵抗和腫瘤等病理過程密切相關。 |
| 產品圖片 |
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-ADFP (bs-1164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADFP Polyclonal Antibody, Unconjugated(bs-1164R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
