| 產(chǎn)品編號 | bsm-0391M |
| 英文名稱 | CRP(C11F2) Mouse mAb |
| 中文名稱 | C-反應蛋白單克隆抗體 |
| 別 名 | C-reactive protein; C Reactive Protein; C reactive protein pentraxin related; CRP; MGC88244; PTX 1; PTX1; C-reactive protein(1-205); CRP_HUMAN; Pentraxin 1. |
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Specific References (3) | bsm-0391M has been referenced in 3 publications.
[IF=15.881] Maoze Guo. et al. Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management. Acs Nano. 2021;15(9):15316–15327 Other ; material.
[IF=11.092] Baoyang Lin. et al. Patterned Duplex Fibric based on Genetically Modified Spidroin for Smart Wound Management. ADV HEALTHC MATER. 2022 Nov;:2202213 IA ; Mouse.
[IF=0.5] Zhou, Mingxue, et al. "The effect of hydroxy safflower yellow A on inflammatory reaction in myocardium of the rats after acute myocardial infarction." African Journal of Pharmacy and Pharmacology 7.12 (2013): 643-649. IHC-P ; Rat.
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| 研究領域 | 心血管 染色質和核信號 信號轉導 細胞凋亡 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | C11F2 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinded CRP protein |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture. Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Subunit: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. Subcellular Location: Secreted. Tissue Specificity: Found in plasma. Similarity: Belongs to the pentaxin family. Contains 1 pentaxin domain. Database links: Entrez Gene: 1401 Human Omim: 123260 Human SwissProt: P02741 Human Unigene: 76452 Human C-反應蛋白-CRP（C-reactive protin）是與C性肺炎球菌的多糖起反應的一種蛋白，在某些細菌的感染過程中出現(xiàn)在血漿中。 |
| 產(chǎn)品圖片 |
Sample:
C Reactive Protein(CRP) Lysate at 40 ug
Primary: Anti- CRP(C11F2) (bsm-0391M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27 kD
Sample:
Lane 1: U2os (Human) Cell Lysate at 30 ug
Lane 2: Siha (Human) Cell Lysate at 30 ug
Primary:
Anti- CRP (bsm-0391M) at 1/1000 dilution
Anti- beta-Actin (bs-0061R) at 1/2000 dilution
Secondary:
IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 26 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Polyclonal Antibody, Unconjugated (bsm-0391M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Monoclonal Antibody, Unconjugated (ascites of bsm-0391M C11F2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Monoclonal Antibody, Unconjugated (ascites of bsm-0391M C11F2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
