| 產(chǎn)品編號 | bs-1569R |
| 英文名稱 | MCT1/Malignant T cell amplified sequence 1 Rabbit pAb |
| 中文名稱 | 單羧酸轉(zhuǎn)運蛋白-1抗體 |
| 別 名 | monocarboxylate transporter 1; Malignant T cell amplified sequence 1; MCT 1; MCT1; MCTS 1; MCTS1; Oncogene MCT 1; Oncogene MCT1; SLC16A1; FLJ36745; HHF7; MCT; MGC44475; monocarboxylate transporter; Monocarboxylate transporter isoform 1; Monocarboxylic acid transporter 1; SLC16A1; SLC16A1 protein; Solute carrier family 16(monocarboxylic acid transporters) member 1; Solute carrier family 16 member 1(monocarboxylic acid transporter 1); Solute carrier family 16 member 1; MOT1_HUMAN. |
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Specific References (1) | bs-1569R has been referenced in 1 publications.
[IF=0] Klasvogt, Sonja, et al. "Air–liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2." Cell Death Discovery 3 (2017): 17001. WB ; Pig.
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| 研究領(lǐng)域 | 免疫學(xué) 轉(zhuǎn)運蛋白 交換蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MCT1: 34-59/500 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009] Function: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed in normal and in cancer cells. DISEASE: Defects in SLC16A1 are the cause of symptomatic deficiency in lactate transport (SDLT) [MIM:245340]; also known as erythrocyte lactate transporter defect. Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals. Defects in SLC16A1 are the cause of familial hyperinsulinemic hypoglycemia type 7 (HHF7) [MIM:610021]; also known as exercise-induced hyperinsulinemic hypoglycemia. HHF7 is a dominantly inherited hypoglycemic disorder characterized by inappropriate insulin secretion during anaerobic exercise or on pyruvate load. Similarity: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. SWISS: P53985 Gene ID: 6566 Database links: Entrez Gene: 6566 Human Entrez Gene: 20501 Mouse Omim: 600682 Human SwissProt: P53985 Human SwissProt: P53986 Mouse Unigene: 75231 Human Unigene: 9086 Mouse 單羧酸轉(zhuǎn)運蛋白家族是哺乳動物細胞膜上一類重要的跨膜轉(zhuǎn)運蛋白,負責(zé)乳酸、短鏈脂肪酸等單羧酸類化合物的跨膜轉(zhuǎn)運,有促進營養(yǎng)物質(zhì)吸收、代謝動態(tài)的平衡、調(diào)節(jié)胞內(nèi)pH值以及參與藥物輸送等多種生物學(xué)功能。 |
| 產(chǎn)品圖片 |
Sample:
Brain(Rat) lysate at 45ug;
Colon carcinoma(Human) lysate at 45ug;
Primary: Anti-MCT1 (bs-1569R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
Predicted band size : 55kD
Observed band size : 55kD
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Stomach (Mouse) Lysate at 40 ug
Lane 3: Heart (Mouse) Lysate at 40 ug
Lane 4: Liver (Rat) Lysate at 40 ug
Lane 5: Stomach (Rat) Lysate at 40 ug
Lane 6: Heart (Rat) Lysate at 40 ug
Primary: Anti-MCT1 (bs-1569R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated(bs-1569R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated(bs-1569R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
