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DFFA Rabbit pAb (bs-0214R)  
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產(chǎn)品編號 bs-0214R
英文名稱 DFFA Rabbit pAb
中文名稱 DFFA抗體
別    名 DNA fragmentation factor subunit alpha; DFF-45; Inhibitor of CAD; ICAD; DFF1; DFF45; H13; DFFA; DFFA_HUMAN;  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號  細(xì)胞凋亡  新陳代謝  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 36 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ICAD: 2-100/331 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Caspase-activated deoxyribonuclease(CAD) also designated DNA fragmentation factor 45 kDa subunit (DFF-45)and Caspase-activated DNase. CAD is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. ICAD (DNA fragmentation factor alpha subunit) (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) is inhibitor of the caspase-activated DNase (DFF44).

Function:
Inhibitor of the caspase-activated DNase (DFF40).

Subunit:
Heterodimer of DFFA and DFFB.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.

Similarity:
Contains 1 CIDE-N domain.

SWISS:
O00273

Gene ID:
1676

Database links:

Entrez Gene: 1676 Human

Omim: 601882 Human

SwissProt: O00273 Human

Unigene: 484782 Human



ICAD 是在細(xì)胞凋亡期誘導(dǎo)DNA分裂和染色體固縮的一種核酶,降解裸露的DNA 并誘導(dǎo)形態(tài)學(xué)變化。
產(chǎn)品圖片
Sample: liver (mouse) Lysate at 40 ug lung(mouse) Lysate at 40 ug BRL-3A (rat)cell Lysate at 40 ug Primary: Anti- ICAD (bs-0214R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36kD Observed band size: 36 kD
Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-ICAD (bs-0214R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ICAD Polyclonal Antibody, Unconjugated(bs-0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ICAD/DFF-45 Beta Polyclonal Antibody, Unconjugated(bs-0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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