| 產(chǎn)品編號 | bsm-0388M |
| 英文名稱 | IFN gamma(F2E4) Mouse mAb |
| 中文名稱 | γ-干擾素/γ-IFN單克隆抗體 |
| 別 名 | IFNG; IFG; IFI; IFN gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon; hIFN-gamma. |
 |
Specific References (1) | bsm-0388M has been referenced in 1 publications.
[IF=3.17] Zhang, Jia‐xiang, et al. "Complement C5a–C5aR interaction enhances MAPK signaling pathway activities to mediate renal injury in trichloroethylene sensitized BALB/c mice." Journal of Applied Toxicology (2015). IHC-P ; Mouse.
|
| 研究領(lǐng)域 | 腫瘤 免疫學 細胞因子 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | F2E4 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1ug/Test,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Full length of human IFN gamma: 24-161/166 (C-6x His-Tag) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2. Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Released primarily from activated T ymphocytes. Post-translational modifications: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. DISEASE: In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. Similarity: Belongs to the type II (or gamma) interferon family. SWISS: P01579 Gene ID: 3458 Database links: Entrez Gene: 3458 Human Omim: 147570 Human SwissProt: P01579 Human Unigene: 856 Human |
| 產(chǎn)品圖片 |
4% Paraformaldehyde-fixed Jurkat（Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 μg/mL, 6 h), BFA (5 μg/ml, last 5 h)）(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IFN gamma) monoclonal Antibody, unconjugated (bsm-0388M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Jurkat（Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 μg/mL, 6 h), BFA (5 μg/ml, last 5 h)） (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-IFN gamma antibody (bsm-0388M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
