| 產品編號 | bs-0099R |
| 英文名稱 | Rabbit Anti-TGF beta 3 propeptide antibody |
| 中文名稱 | 轉移生長因子β3抗體（TGFβ3） |
| 別 名 | Transforming growth factor beta-3 proprotein; TGFB3; ARVD; FLJ16571; MGC105479; MGC118722; TGF beta 3; TGF beta3; TGF-beta-3; TGF-beta 3; TGFB 3; Transforming growth factor beta-3; TGFB3_HUMAN. |
| 研究領域 | 信號轉導 干細胞 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat,Pig (predicted: Rabbit,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TGF-beta 3: 41-90/412 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The TGFBeta superfamily is composed of numerous growth and differentiation factors, including transforming growth factors Beta(TGFBeta) 1,2 and 3; placental transforming growth factor (PTGFBeta);growth /differentiation factors (GDFs); mullerian inhibiting substance (MIS);bone morphogenic proteins (BMPs);glial cell line-derived neurotrophic factor (GDNF);inhibins or activins (Alpha,Beta-A, Beta-B and Beta-C),Lefty and Nodal. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis. Function: Involved in embryogenesis and cell differentiation. Subunit: Homodimer; disulfide-linked. Interacts with ASPN. Subcellular Location: Secreted. DISEASE: Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Similarity: Belongs to the TGF-beta family. SWISS: P10600 Gene ID: 7043 Database links: Entrez Gene: 7043 Human Entrez Gene: 21809 Mouse Omim: 190230 Human SwissProt: P10600 Human SwissProt: P17125 Mouse Unigene: 592317 Human Unigene: 3992 Mouse Unigene: 7018 Rat 生長因子和激素（ Growth Factor and Hormones） TGF是一種多效生長因子,對上皮細胞增值有潛在抑制作用,可抑制腫瘤生長，用于許多惡性腫瘤如:胃癌、肺癌、膀胱癌、腎癌、前列腺癌、結腸癌等多種惡性腫瘤的研究。分子量：47kDa。此抗體是TGFβ3 特異性抗體，與TGF-β1和TGF-β2沒有交叉反應。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Prostate tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Rat Prostate tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human MCF-7 cell lysates
Lane 7: Human SH-SY5Y cell lysates
Primary: Anti-TGF beta 3 propeptide (bs-0099R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30/47 kDa
Observed band size: 47 kDa
Tissue/cell: pig bile duct; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta-3 Polyclonal Antibody, Unconjugated(bs-0099R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: SHSY5Y.
Primary Antibody (green line): Rabbit Anti-TGF beta 3 propeptide antibody (bs-0099R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
