| 產(chǎn)品編號 | bs-0272R |
| 英文名稱 | Rabbit Anti-Visfatin antibody |
| 中文名稱 | 內(nèi)脂素/內(nèi)臟脂肪素/前B細胞克隆增強因子1抗體 |
| 別 名 | pre-B-cell colony-enhancing factor 1; PBEF1 protein; AI480535; 1110035O14Rik; AI314458; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre B cell colony enhancing factor 1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; Visfatin; NAMPT_HUMAN. |
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Specific References (2) | bs-0272R has been referenced in 2 publications.
[IF=5.032] Cai, Bolin. et al. circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis. J Anim Sci Biotechno. 2022 Dec;13(1):1-15 WB ; Chicken.
[IF=1.77] Lin, Li-Wen, et al. "Visfatin/pre-B cell colony-enhancing factor immunohistochemical overexpression in oral cancers." Journal of Applied Biomedicine (2014). IHC-P ; Human.
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| 研究領域 | 心血管 神經(jīng)生物學 糖尿病 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Chicken,Horse,Monkey) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PBEF: 301-400/491 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]. Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Subunit: Homodimer. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. Similarity: Belongs to the NAPRTase family. SWISS: P43490 Gene ID: 10135 Database links: Entrez Gene: 10135 Human Entrez Gene: 59027 Mouse Omim: 608764 Human SwissProt: P43490 Human SwissProt: Q99KQ4 Mouse Unigene: 489615 Human Unigene: 202727 Mouse Unigene: 203508 Rat 一種新的脂肪因子-visfatin是新近發(fā)現(xiàn)的主要由人和小鼠內(nèi)臟脂肪組織分泌的一種脂肪細胞因子,其結(jié)構(gòu)與pre-B細胞集落增強因子相似.它能夠發(fā)揮類似胰島素的作用,與Ⅱ型糖尿病相關聯(lián),降低血糖,促進糖攝取,可結(jié)合并活化胰島素受體,激活胰島素信號通路.內(nèi)臟脂肪因子-Visfatin與肥胖密切相關并能夠促進脂肪細胞的分化,還能促進血管平滑肌細胞成熟. Visfatin的表達受炎癥反應因子和多種激素的調(diào)節(jié).PBEF-1可能是聯(lián)系機體糖脂代謝的重要分子,它的發(fā)現(xiàn)可為揭示糖尿病與肥胖的發(fā)生發(fā)展機制提供新的研究思路,為代謝綜合征的治療提供新方案. |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-Visfatin (bs-0272R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Lane1:Brain(Rat) Lysate at 30 ug
Lane2:Liver (Rat) Lysate at 30 ug
Primary: Anti-VISFATIN-1 (bs-0272R) at 1:200 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution
Predicted band size : 55kD
Observed band size : 54kD
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Visfatin) Polyclonal Antibody, Unconjugated (bs-0272R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(bs-0272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(bs-0272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Visfatin antibody (bs-0272R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
