| 產(chǎn)品編號 | bsm-2011M |
| 英文名稱 | BCHE(1E8) Mouse mAb |
| 中文名稱 | 丁酰膽堿酯酶單克隆抗體 |
| 別 名 | Cholinesterase; Acylcholine acylhydrolase; Choline esterase II; CHE1; Butyrylcholine esterase; Pseudocholinesterase; butyrylcholinesterase.CHLE_HUMAN. |
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Specific References (1) | bsm-2011M has been referenced in 1 publications.
[IF=6.59] Jun Li. et al. Therapeutic targets and functions of curcumol against COVID-19 and colon adenocarcinoma. FRONT NUTR. 2022; 9: 961697 IHC ; Human.
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| 研究領(lǐng)域 | 心血管 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 1E8 |
| 交叉反應 | Rat (predicted: Human) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 66 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Human BCHE native protein purified |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008]. Function: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Subunit: Homotetramer; disulfide-linked. Dimer of dimers. Subcellular Location: Secreted. Tissue Specificity: Detected in blood plasma (at protein level). Present in most cells except erythrocytes. DISEASE: Butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]: Metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the type-B carboxylesterase/lipase family. SWISS: P06276 Gene ID: 590 Database links: Entrez Gene: 590 Human Omim: 177400 Human SwissProt: P06276 Human Unigene: 420483 Human 血清丁酰膽堿酯酶是由四個亞基組成的糖蛋白，分子量28萬～35萬。作為一類糖蛋白，主要存在于腦、肝、血及膽堿能神經(jīng)末稍的突觸間隙中，主要由肝臟合成，并存在于血清中，生理作用不明，稱為假性膽堿酯酶或丁酰膽堿酯酶[1]Lockridge等采用Edman降解法測定了人血清BChE完整的氨基酸序列，共含有574個氨基酸殘基。BChE氨基酸序列與其他酯酶家族蛋白有很高的同源性。以電鰩AChE的三維結(jié)構(gòu)為模板對人BChE的三維結(jié)構(gòu)進行計算機模擬結(jié)果表明，BChE活性中心，囊袋的底部為Ser198，其兩側(cè)相距一定距離分別是?；Y(jié)合部位和膽堿結(jié)合部位。研究發(fā)現(xiàn)人BChE的氨基酸序列上有Zn2+離子結(jié)合結(jié)構(gòu)。 |
| 產(chǎn)品圖片 |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation:Anti-BCHE(1E8) Y Polyclonal Antibody, Unconjugated(bsm-2011M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
