| 產(chǎn)品編號(hào) | bs-2516R |
| 英文名稱 | GTSE1 Rabbit pAb |
| 中文名稱 | G2期/S期應(yīng)答相關(guān)蛋白1抗體 |
| 別 名 | G2 and S phase-expressed protein 1; B99; B99 homolog; G 2 and S phase expressed 1; G two S phase expressed protein 1; GTSE 1; GTSE-1; GTSE1_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow,Chicken) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 77 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GTSE1: 651-739/739 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis. [provided by RefSeq, Jul 2008] Function: May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression. Subcellular Location: Cytoplasm, cytoskeleton. Note=Associated with microtubules. Post-translational modifications: Phosphorylated in mitosis. Similarity: Sequence=CAQ09324.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=CAQ10552.2; Type=Erroneous initiation; Note=Translation N-terminally shortened. SWISS: Q9NYZ3 Gene ID: 51512 Database links: Entrez Gene: 51512 Human Omim: 607477 Human SwissProt: Q9NYZ3 Human |
| 產(chǎn)品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti- GTSE-1 (bs-2516R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 75 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GTSE-1) Polyclonal Antibody, Unconjugated (bs-2516R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GTSE-1 Polyclonal Antibody, Unconjugated(bs-2516R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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