| 產(chǎn)品編號 | bs-4030R |
| 英文名稱 | Rabbit Anti-Pancreatic Amylase antibody |
| 中文名稱 | 胰淀粉酶抗體 |
| 別 名 | Alpha amylase; Alpha amylase pancreatic precursor; AMY2; AMY2A; AMY2B; Amylase alpha 2A; Amylase alpha 2A pancreatic; Amylase alpha 2B; Amylase alpha 2B pancreatic; Amylase pancreatic alpha 2A; Amylase pancreatic alpha 2B; Pancreatic alpha amylase; AMYP_HUMAN. |
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Specific References (1) | bs-4030R has been referenced in 1 publications.
[IF=9.685] Peng, Cheng. et al. MLKL signaling regulates macrophage polarization in acute pancreatitis through CXCL10. CELL DEATH DIS. 2023 Feb;14(2):1-14 IF ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56kDa |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pancreatic Amylase: 151-250/511 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa.Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis). Subunit: Monomer. Subcellular Location: Secreted, extracellular space. Similarity: Belongs to the glycosyl hydrolase 13 family. SWISS: P04746 Gene ID: 279 Database links: Entrez Gene: 279 Human Entrez Gene: 280 Human Entrez Gene: 545562 Mouse Omim: 104650 Human Omim: 104660 Human SwissProt: P04746 Human SwissProt: P19961 Human SwissProt: P00688 Mouse Unigene: 484588 Human Unigene: 654437 Human ????胰淀粉酶由胰腺以活性狀態(tài)排入消化道，是最重要的水解碳水化合物的酶，和唾液腺分泌的淀粉酶一樣都屬于α-淀粉酶，作用于α-1，4糖苷鍵，對分支上的α-1，6糖苷鍵無作用，故又稱淀粉內(nèi)切酶，其作用的最適pH為6.9，可通過腎小球濾過，是唯一能在正常時于尿中出現(xiàn)的血漿酶。 ????人體的其他組織如卵巢、輸卵管、肺、睪丸、精液、乳腺等的提取物中都發(fā)現(xiàn)有淀粉酶活性;血液、尿液、乳液中也含淀粉酶。血液淀粉酶中主要來自胰腺、唾液腺，尿液中淀粉酶則來自于血液.。當罹患胰腺疾病，或有胰腺外分泌功能障礙時都可引起其活性升高或降低，有助于胰腺疾病的診斷。尿淀粉酶水平波動較大，所以用血清淀粉酶檢測為好，或兩者同時測定。淀粉酶活性變化亦可見于某些非胰腺疾患。 |
| 產(chǎn)品圖片 |
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-Pancreatic Amylase (bs-4030R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Pancreatic Amylase (bs-4030R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pancreatic Amylase) Polyclonal Antibody, Unconjugated (bs-4030R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
