| 產(chǎn)品編號 | bs-4059R |
| 英文名稱 | Rabbit Anti-FABP4 antibody |
| 中文名稱 | 脂肪細胞型脂肪酸結(jié)合蛋白抗體 |
| 別 名 | adipocyte; 3T3-L1 lipid-binding protein; 422/aP2; A FABP; A-FABP; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; Adipocyte protein AP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBPv ALBP/Ap2; aP2; FABP; FABP4_HUMAN; Fatty acid binding protein 4 adipocyte; Fatty acid binding protein 4; Fatty acid binding protein adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein; Lbpl; Myelin P2 protein homolog; P15v P2 adipocyte protein; Protein 422. |
 |
Specific References (4) | bs-4059R has been referenced in 4 publications.
[IF=27.287] Clair Crewe. et al. Extracellular vesicle-based interorgan transport of mitochondria from energetically stressed adipocytes. Cell Metab. 2021 Sep;33:1853 FC ; Mouse.
[IF=3.79] Claycombe, Kate J., et al. "Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (9Ga) and reduced FGF21 gene expression in Sprague Dawley rats fed prenatal low protein and postnatal high fat diets." The Journal of Nutritional Biochemistry (2016). FCM ; Rat.
[IF=3.123] Ferrante et al. Adipocyte-derived exosomal miRNAs: a novel mechanism for obesity-related disease. (2015) Pediatr.Res. 77:447-54 FCM ; Human.
[IF=1.399] Haodong Liu. et al. miR‐340‐5p inhibits sheep adipocyte differentiation by targeting ATF7. Anim Sci J. 2020 Jan;91(1):e13462 WB ; Sheep.
|
| 研究領(lǐng)域 | 腫瘤 免疫學 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FABP4: 61-132/132 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq]. Function: Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Subunit: Monomer. Homodimer. Interacts with PPARG. Subcellular Location: Cytoplasm. Nucleus. Note=Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. Similarity: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. SWISS: P15090 Gene ID: 2167 Database links: Entrez Gene: 2167 Human Entrez Gene: 11770 Mouse Omim: 600434 Human SwissProt: P15090 Human SwissProt: P04117 Mouse Unigene: 391561 Human Unigene: 582 Mouse Unigene: 4258 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FABP4) Polyclonal Antibody, Unconjugated (bs-4059R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
