| 產(chǎn)品編號 | bs-2946R |
| 英文名稱 | Rabbit Anti-HAS1 antibody |
| 中文名稱 | 透明質(zhì)酸合成酶1抗體 |
| 別 名 | HA synthase 1; HAS; HAS1; HAS 1; HAS-1; HAS1_HUMAN; HuHAS1; Hyaluronan synthase 1; Hyaluronan synthase; HA Synthase: Hyaluronate synthase 1; Hyaluronic acid synthase 1. |
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Specific References (2) | bs-2946R has been referenced in 2 publications.
[IF=49.962] Pascual, Gloria. et al. Dietary palmitic acid promotes a prometastatic memory via Schwann cells. Nature. 2021 Nov;:1-6 IF ; Mouse.
[IF=2.22] Yang, Guofeng, et al. "Hyaluronan and hyaluronan synthases expression and localization in embryonic mouse molars." Journal of Molecular Histology: 1-8. IHC-P ; Mouse.
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| 研究領域 | 心血管 細胞生物 發(fā)育生物學 神經(jīng)生物學 信號轉(zhuǎn)導 干細胞 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hyaluronan synthase 1: 501-578/578 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HAS1, HAS2 and HAS3 are HA Synthase proteins that synthesize HA (Hyaluronan or hyaluronic acid). The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by beta-1,3 and beta-1,4 glycosidic bonds. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. Both HAS1 and HAS2 synthesize high molecular weight HA, whereas HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli, suggesting distinct functional roles of the three proteins. HAS1 mRNA shows predominant expression in bone marrow mesenchymal progenitor cells and synovial cells. Function: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate. Subcellular Location: Membrane; Multi-pass membrane protein Tissue Specificity: Highly expressed in ovary followed by spleen, thymus, prostate, testes and large intestine. Weakly expressed in small intestine. Similarity: Belongs to the NodC/HAS family. SWISS: Q92839 Gene ID: 3036 Database links: Entrez Gene: 3036 Human Omim: 601463 Human SwissProt: Q92839 Human Unigene: 57697 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Testis Lysates
Lane 2: Mouse Spleen Lysates
Lane 3: Rat Testis Lysates
Lane 4: Rat Spleen Lysates
Lane 5: Human U-87 MG cell Lysates
Lane 6: Human DU145 cell Lysates
Primary: Anti-HAS1 (bs-2946R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65kDa
Observed band size: 65kDa
Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HAS1 Polyclonal Antibody, Unconjugated(bs-2946R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Black line : Positive blank control (U87MG); Negative blank control (A431）
Green line : Primary Antibody (Rabbit Anti-HAS1 antibody (bs-2946R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
U87MG（Positive）and A431（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HAS1 Antibody(bs-2946R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: mouse thymouses(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
