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TNMD Rabbit pAb (bs-7525R)  
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50ul/1180.00元
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產(chǎn)品編號(hào) bs-7525R
英文名稱(chēng) TNMD Rabbit pAb
中文名稱(chēng) 腱調(diào)蛋白/軟骨調(diào)節(jié)素樣1蛋白抗體
別    名 BRICD4; BRICHOS domain containing 4; CHM1 LIKE; CHM1L; Chondromodulin I like; Chondromodulin I like protein; Chondromodulin-1-like protein; Chondromodulin-I-like protein; hChM1L; hTeM; Myodulin; TEM; Tendin; Tenomodulin; Tenomodulin protein; TNMD; TNMD_HUMAN; UNQ771/PRO1565.  
Specific References  (11)     |     bs-7525R has been referenced in 11 publications.
[IF=10.273] Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.  ICF ;  Rabbit.  
[IF=7.01] Xin Wang. et al. Irreversible Electroporation Improves Tendon Healing in a Rat Model of Collagenase-Induced Achilles Tendinopathy. AM J SPORT MED. ;():  IHC,IF ;  Rat.  
[IF=5.88] Shuai Chen. et al. Tenogenic adipose-derived stem cell sheets with nanoyarn scaffolds for tendon regeneration. Mat Sci Eng C-Mater. 2021 Feb;119:111506  WB,IHC ;  Rabbit.  
[IF=5.131] Kang Lu. et al. Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the α2β1/FAK/PI3K/AKT Signaling Pathway In Vitro. STEM CELLS INT. 2023 Jan 13;2023:2915826  IHC ;  Rat.  
[IF=3.869] Chen Guanyin. et al. Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits. Stem Cells Int. 2020;2020:8822609  IF,WB,IHC ;  Rabbit.  
[IF=3.651] Han Wang. et al. Introduction of tenomodulin by gene transfection vectors for rat bone tissue regeneration. REGEN THER. 2023 Mar;22:99  IHC ;  Rat.  
[IF=3.063] Zhou YC et al. Effect of book-shaped acellular tendon scaffold with bone marrow mesenchymal stem cells sheets on bone–tendon interface healing. Chem Biol Interact. 2020 May 25;323:109074.  IF ;  rabbit.  
[IF=2.93] Wada, Satoshi, et al. "H3K9MTase G9a is essential for the differentiation and growth of tenocytes in vitro." Histochemistry and Cell Biology (2015): 1-8.  IHC-P ;  Mouse.  
[IF=2.813] Lu, Kang. et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling. BMC MOL CELL BIOL. 2023 Dec;24(1):1-13  IF,IHC ;  Rat.  
[IF=2.61] Shimada, Akemi, et al. "Efficient expansion of mouse primary tenocytes using a novel collagen gel culture method." Histochemistry and Cell Biology (2014): 1-11.  Mouse.  
[IF=1.798] Kai KANG. et al. Lrrc32 accelerates tenogenic differentiation of tendon-derived stem cells and promotes Achilles tendon repair in rats. EXP ANIM TOKYO. 2022 Aug 05  IF ;  Rat.  
研究領(lǐng)域 心血管  血管內(nèi)皮細(xì)胞  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 37 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TNMD: 151-250/317 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein that is related to chondromodulin-I, which is a cartilage-specific glycoprotein that functions to stimulate chondrocyte growth and to inhibit tube formation of endothelial cells. This protein is also an angiogenesis inhibitor. Genetic variation in this gene is associated with a risk for type 2 diabetes, central obesity and serum levels of systemic immune mediators in a body size-dependent manner. This gene is also a candidate gene for age-related macular degeneration, though a direct link has yet to be demonstrated. [provided by RefSeq, Sep 2009].

Function:
May be an angiogenesis inhibitor.

Subcellular Location:
Membrane; Single-pass type II membrane protein.

Similarity:
Belongs to the chondromodulin-1 family.
Contains 1 BRICHOS domain.

SWISS:
Q9H2S6

Gene ID:
64102

Database links:

Entrez Gene: 64102 Human

Entrez Gene: 64103 Mouse

Entrez Gene: 64104 Rat

Omim: 300459 Human

SwissProt: Q9H2S6 Human

SwissProt: Q9EP64 Mouse

SwissProt: Q9ESC2 Rat

Unigene: 132957 Human

Unigene: 46221 Mouse

Unigene: 26691 Rat



產(chǎn)品圖片
Sample: Lane 1: Muscle (Rat) Lysate at 40 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: SW480 (Human) Cell Lysate at 30 ug Primary: Anti- TNMD (bs-7525R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 35 kD
Sample: Lane 1: Skeletal muscle (Rat) Lysate at 40 ug Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti-TNMD (bs-7525R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human mammary gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TNMD/tenomodulin Polyclonal Antibody, Unconjugated(bs-7525R) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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