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TNIP2 Rabbit pAb (bs-7563R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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產(chǎn)品編號(hào) bs-7563R
英文名稱(chēng) TNIP2 Rabbit pAb
中文名稱(chēng) 腫瘤壞死因子α誘導(dǎo)蛋白3相互作用蛋白2抗體
別    名 A20 binding inhibitor of NF kappaB activation 2; ABIN 2; FLIP1; KLIP; LKB1 interacting protein; TNFAIP3 interacting protein 2; TNIP2_MOUSE.  
研究領(lǐng)域 心血管  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  內(nèi)皮細(xì)胞  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Rat (predicted: Mouse,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 49 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TNIP2/ABIN2/TNFAIP3 interacting protein 2: 85-180/430 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The human TNIP2 (also known as ABIN-2), which is related to proteins ABIN-1 and ABIN-3, was originally identified as an A20-associating cytosolic protein that block nuclear factor kappaB (NF-kB) activation. NF-kB plays a central role in the regulation of genes implicated in immune responses, inflammatory processes, and apoptotic cell death and the zinc finger protein A20 is a potent inhibitor of NF-kB activity with a key role in limiting the extent and duration of inflammatory activation. It was also reported that ABIN-2 has the potential to enter the nucleus and plays a role in mediating transcriptional activation in both yeast and mammalian cells.

Function:
Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. Involved in activation of the MEK/ERK signaling pathway during innate immune response; this function seems to be stimulus- and cell type specific. Required for stability of MAP3K8. Involved in regulation of apoptosis in endothelial cells; promotes TEK agonist-stimulated endothelial survival. May act as transcriptional coactivator when translocated to the nucleus. Enhances CHUK-mediated NF-kappa-B activation involving NF-kappa-B p50-p65 and p50-c-Rel complexes.

Subunit:
nteracts with STK11/LKB1, TNFAIP3, IKBKG, NFKB1, MAP3K8, TEK, RIPK1, CHUK, IKBKB and SMARCD1. Interacts with polyubiquitin

Tissue Specificity:
Ubiquitously expressed in all tissues examined.

Post-translational modifications:
In vitro phosphorylated by CHUK (By similarity).
Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination probably leading to constitutive proteasomal degradation which can be impaired by IKK-A/CHUK or IKBKB probbaly involving deubiquitination (By similarity).

SWISS:
Q8NFZ5

Gene ID:
79155

Database links:

Entrez Gene: 79155 Human

Entrez Gene: 231130 Mouse

Omim: 610669 Human

SwissProt: Q8NFZ5 Human

SwissProt: Q99JG7 Mouse

Unigene: 726088 Human

Unigene: 28615 Mouse



產(chǎn)品圖片
Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-TNIP2 (bs-7563R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 49 kD Observed band size: 49 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TNIP2 Polyclonal Antibody, Unconjugated(bs-7563R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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