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BMP1 Rabbit pAb (bs-6401R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-6401R
英文名稱 BMP1 Rabbit pAb
中文名稱 骨形態(tài)發(fā)生蛋白1/膠原C蛋白肽鏈內(nèi)切酶抗體
別    名 BMP 1; BMP-1; Bone morphogenetic protein 1; Mammalian tolloid protein; mTld; PCOLC; PCP; ProCollagen C endopeptidase; Procollagen C proteinase; TLD; BMP1_HUMAN.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  細(xì)胞骨架  細(xì)胞外基質(zhì)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 98 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞外基質(zhì) 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BMP1: 901-986/986 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Bone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved in extracellular matrix (ECM) formation, suggesting that a functional link may exist between astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during development. The name PCP reflects this enzyme’s involvement in the collagen deposition of growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are involved in the processing of fibrillar procollagen precursors to mature collagens, which is an essential requirement for fibril formation. PCP cleaves the C-terminus from procollagen, to allow the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen N-proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at the C-terminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have extra EGF-like and CUB domains.

Function:
Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD).

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the peptidase M12A family. Contains 5 CUB domains. Contains 2 EGF-like domains.

SWISS:
P13497

Gene ID:
649

Database links:

Entrez Gene: 649 Human

Entrez Gene: 12153 Mouse

Entrez Gene: 83470 Rat

Omim: 112264 Human

SwissProt: P13497 Human

SwissProt: P98063 Mouse

Unigene: 1274 Human

Unigene: 27757 Mouse

Unigene: 9305 Rat



產(chǎn)品圖片
Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-BMP1 (bs-6401R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 98 kD
Sample: Lane 1: Mouse Placenta tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Mouse NIH/3T3 cell lysates Lane 4: Rat Placenta tissue lysates Lane 5: Human HeLa cell lysates Lane 6: Human A549 cell lysates Primary: Anti-BMP1 (bs-6401R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kDa Observed band size: 100 kDa
Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BMP1 Polyclonal Antibody, Unconjugated(bs-6401R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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