| 產品編號 | bs-9522R |
| 英文名稱 | MPP1 Rabbit pAb |
| 中文名稱 | 紅細胞膜蛋白55抗體 |
| 別 名 | palmitoylated 1; 55 kDa erythrocyte membrane protein; AAG 12; AAG12; Aging associated gene 12; DXS552; DXS552E; EM55_HUMAN; EMP 55; EMP55; EMP-55; Erythrocyte membrane protein p55; Membrane protein; Membrane protein palmitoylated 1 55kDa; Membrane protein |
| 研究領域 | 心血管 細胞生物 免疫學 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Dog) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MPP1/EMP55: 101-200/466 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The Kinesins constitute a large family of microtubule-dependent motor proteins which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual Kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events, including endocytosis and transcytosis. MPP1 (M-phase phosphoprotein 1), also known as KIF20B (kinesin family member 20B), MPHOSPH1 or KRMP1, is a 1,820 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one kinesin-motor domain. Expressed in kidney, brain, testis and ovary, MPP1 functions as a plus-end directed motor enzyme that interacts with Pin1 and is required for the completion of cytokinesis. MPP1, which exists as multiple alternatively spliced isoforms termed 1-5, is subject to post-translational phosphorylation, probably by ATM or ATR. Function: Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity). Subunit: Heterodimer with MPP5. Interacts with DLG5 and NF2. Interacts (via guanylate kinase-like domain) with WHRN (via third PDZ domain). Subcellular Location: Membrane; Lipid-anchor. Cell projection, stereocilium (By similarity). Note=Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). Colocalizes with NF2 in non-myelin-forming Schwann cells. Tissue Specificity: Ubiquitous. Post-translational modifications: Extensively palmitoylated by ZDHHC17, palmitoylation is essential for membrane organization and is crucial for proper erythrocytes morphology. Similarity: Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 1 PDZ (DHR) domain. Contains 1 SH3 domain. SWISS: Q00013 Gene ID: 4354 Database links: Entrez Gene: 4354 Human Entrez Gene: 17524 Mouse Entrez Gene: 100328768 Rabbit Omim: 305360 Human SwissProt: Q00013 Human SwissProt: P70290 Mouse Unigene: 496984 Human Unigene: 391267 Mouse |
| 產品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- MPP1 (bs-9522R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti- MPP1 (bs-9522R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
