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Tropomyosin Rabbit pAb (bs-9622R)  
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產(chǎn)品編號(hào) bs-9622R
英文名稱 Tropomyosin Rabbit pAb
中文名稱 原肌球蛋白1抗體
別    名 Tropomyosin 1(alpha); alpha tropomyosin; alpha-tropomyosin; C15orf13; cardiomyopathy, hypertrophic 3; CMD1Y; CMH3; HTM alpha; sarcomeric tropomyosin kappa; TMSA; TPM1; TPM1_HUMAN; Tropomyosin 1; Tropomyosin1; Tropomyosin alpha 1 chain; Tropomyosin alpha-1 chain; Tropomyosin-1; Tropomyosin α; Tropomyosin-α; Tropomyosinα.  
研究領(lǐng)域 心血管  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞骨架  細(xì)胞外基質(zhì)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human (predicted: Mouse,Rat,Sheep,Zebrafish,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 33 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Tropomyosin: 201-284/284 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Function:
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Subunit:
Heterodimer of an alpha and a beta chain (By similarity). Interacts with HRG (via the HRR domain); the interaction contributes to the antiangiogenic properties of the histidine/proline-rich region (HRR) of HRG.

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

Post-translational modifications:
Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.

DISEASE:
Defects in TPM1 are the cause of familial hypertrophic cardiomyopathy type 3 (CMH3) [MIM:115196]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TPM1 are the cause of cardiomyopathy dilated type 1Y (CMD1Y) [MIM:611878]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Similarity:
Belongs to the tropomyosin family.

SWISS:
P09493

Gene ID:
7168

Database links:

Entrez Gene: 7168 Human

Entrez Gene: 22003 Mouse

Entrez Gene: 100037999 Pig

Entrez Gene: 24851 Rat

Entrez Gene: 30324 Zebrafish

Omim: 191010 Human

SwissProt: Q5KR49 Cow

SwissProt: P09493 Human

SwissProt: P58771 Mouse

SwissProt: P42639 Pig

SwissProt: P04692 Rat

SwissProt: P13104 Zebrafish

Unigene: 133892 Human

Unigene: 121878 Mouse

Unigene: 87540 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human myocardial); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tropomyosin) Polyclonal Antibody, Unconjugated (bs-9622R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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