| 產(chǎn)品編號(hào) | bs-4834R |
| 英文名稱(chēng) | Rabbit Anti-CD73 antibody |
| 中文名稱(chēng) | CD73抗體 |
| 別 名 | NT5; 5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto (CD73); 5'-NT; 5'-nucleotidase; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN antibody eNT; NT antibody NT5; NT5E; NTE; Purine 5 Prime Nucleotidase. |
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Specific References (26) | bs-4834R has been referenced in 26 publications.
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| 研究領(lǐng)域 | 心血管 細(xì)胞生物 免疫學(xué) 細(xì)胞粘附分子 細(xì)胞表面分子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,GuineaPig) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63kDa |
| 檢測(cè)分子量 | 70 |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD73/NT5: 151-250/574 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Subcellular Location: Cell membrane. DISEASE: Defects in NT5E are the cause of calcification of joints and arteries (CAJA). A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. Similarity: Belongs to the 5'-nucleotidase family. SWISS: P21589 Gene ID: 4907 Database links: Entrez Gene: 4907 Human Omim: 129190 Human SwissProt: P21589 Human Unigene: 153952 Human |
| 產(chǎn)品圖片 |
Sample: Kidney (mouse)l Lysate at 40 ug
Primary: Anti- CD73 (bs-4834R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 65 kD
Sample: HepG2 (human)Cell Lysate at 40 ug
Primary: Anti- CD73 (bs-4834R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-CD73 (bs-4834R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63/71 kD
Observed band size: 65 kD
Blank control (blue line): Raji (blue).
Primary Antibody (green line): Rabbit Anti- Phospho-c-Fos (Ser32) antibody (bs-4834R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
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