| 產(chǎn)品編號 | bs-12503R |
| 英文名稱 | Rabbit Anti-APPBP1 antibody |
| 中文名稱 | β淀粉樣蛋白前體蛋白結(jié)合蛋白1抗體 |
| 別 名 | 59 kDa; A 116A10.1; Amyloid beta precursor protein binding protein 1 59kD; Amyloid beta precursor protein binding protein 1 59kDa; Amyloid beta precursor protein binding protein 1; Amyloid beta precursor protein-binding protein 1; Amyloid protein binding protein 1; Amyloid protein-binding protein 1; APP BP1; APP-BP1; APPBP 1; HPP 1; HPP1; NAE 1; NAE1; NEDD8 activating enzyme E1 regulatory subunit; NEDD8 activating enzyme E1 subunit 1; NEDD8 activating enzyme E1 subunit; NEDD8-activating enzyme E1 regulatory subunit; Proto oncogene protein 1; Proto-oncogene protein 1; Protooncogene protein 1; ula 1; ULA1_HUMAN. |
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Specific References (1) | bs-12503R has been referenced in 1 publications.
[IF=2.1] Xiangdong Meng. et al. Naringin ameliorates memory deficits and exerts neuroprotective effects in a mouse model of Alzheimer's disease by regulating multiple metabolic pathways. Mol Med Rep. 2021 May;23(5):1-13 WB ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 表觀遺傳學(xué) 泛素 細(xì)胞膜蛋白 Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APPBP1: 401-500/534 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
APPBP1 is a member of the ubiquitin-activating E1 family. In fetal tissues APPBP1 is widely expressed and in adult tissues it is expressed throughout the brain. APPBP1 is a cell membrane associated protein and functions as the regulatory subunit in a heterodimer with UBA3. The APPBP1/UBA3 complex binds to and activates NEDD8, a ubiquitin-like protein involved in signal transduction, cell proliferation and development. This suggests that APPBP1 affects a variety of cellular functions. In addition, APPBP1 is essential for cell cycle progression through the S/M checkpoint. More specifically, it inhibits the entry into S phase and promotes entry into M phase. Function: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Subunit: Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2. Subcellular Location: Cell membrane. Colocalizes with APP in lipid rafts. Tissue Specificity: Ubiquitous in fetal tissues. Expressed throughout the adult brain. Post-translational modifications: Ubiquitinated by TRIP12, leading to its degradation by the proteasome. Similarity: Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. SWISS: Q13564 Gene ID: 8883 Database links: Entrez Gene: 8883 Human Entrez Gene: 234664 Mouse Omim: 603385 Human SwissProt: Q13564 Human SwissProt: Q8VBW6 Mouse Unigene: 460978 Human Unigene: 237670 Mouse |
| 產(chǎn)品圖片 |
Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-APPBP1 (bs-12503R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APPBP1) Polyclonal Antibody, Unconjugated (bs-12503R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APPBP1) Polyclonal Antibody, Unconjugated (bs-12503R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APPBP1) Polyclonal Antibody, Unconjugated (bs-12503R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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