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HESX1 Rabbit pAb (bs-13597R)  
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產(chǎn)品編號(hào) bs-13597R
英文名稱 HESX1 Rabbit pAb
中文名稱 同源結(jié)構(gòu)域轉(zhuǎn)錄因子HESX1抗體
別    名 HESX1_HUMAN; Homeobox expressed in ES cells 1; Homeobox protein ANF; hAnf.  
研究領(lǐng)域 免疫學(xué)  神經(jīng)生物學(xué)  干細(xì)胞  轉(zhuǎn)錄調(diào)節(jié)因子  細(xì)胞粘附分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 21 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HESX1: 51-150/185 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The homeobox protein, HESX1, which is also known as Rathke’s pouch homeobox, HANF, homeodomain transcription factor, and anterior-restricted homeobox protein is a transcription factor that belongs to the homeodomain family of DNA binding proteins. HESX1 is initially expressed in embryonic stem cells and the primitive forebrain, and is essential for normal development of the eyes and other anterior CNS structures, such as the hypothalamus, the pituitary gland and the olfactory bulbs. The homeobox gene Hesx1 is expressed in the anterior visceral endoderm (AVE), anterior axial mesendoderm (AME), and anterior neural ectoderm (ANE) during early embryogenesis. Mutations in the Hesx1 gene are associated with disorders that are comparable with septo-optic dysplasia (SOD). These disorders are characterized by hypoplasia of the optic nerve, various types of forebrain defects and pituitary hormone deficiencies, including hypothyroidism. Hesx1 also acts as a transcriptional repressor of reporter gene constructs in tissue culture assays.

Function:
Homeobox protein expressed in embryonic stem cells and targetd by Sox2, Oct4 and Nanog. Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor.

Subunit:
Can form heterodimers with PROP1 in binding to DNA. Interacts with TLE1.

Subcellular Location:
Nuclear

DISEASE:
Septooptic dysplasia (SOD) [MIM:182230]: A clinically heterogeneous disorder defined by any combination of optic nerve hypoplasia, pituitary gland hypoplasia with panhypopopituitarism, and midline abnormalities of the brain, including absence of the corpus callosum and septum pellucidum. Note=The disease is caused by mutations affecting the gene represented in this entry.
Growth hormone deficiency with pituitary anomalies (GHDPA) [MIM:182230]: A disease characterized by low or absent growth hormone levels, in the presence of a hypoplastic anterior pituitary lobe and ectopic or absent posterior pituitary lobe. Note=The disease is caused by mutations affecting the gene represented in this entry.
Pituitary hormone deficiency, combined, 5 (CPHD5) [MIM:182230]: Combined pituitary hormone deficiency is defined as the impaired production of growth hormone and one or more of the other five anterior pituitary hormones. CPHD5 is characterized by complete or partial deficiencies of growth hormone, thyroid-stimulating hormone, luteinizing hormone, follicle stimulating hormone and adrenocorticotropic hormone. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the ANF homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
Q9UBX0

Gene ID:
8820

Database links:

Entrez Gene: 8820 Human

Omim: 601802 Human

SwissProt: Q52LC5 Human

SwissProt: Q9UBX0 Human

Unigene: 171980 Human



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HESX1) Polyclonal Antibody, Unconjugated (bs-13597R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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