| 產(chǎn)品編號(hào) | bs-13742R |
| 英文名稱 | CD99L2 Rabbit pAb |
| 中文名稱 | CD99樣蛋白2抗體 |
| 別 名 | C99L2_HUMAN; CD99; CD99 antigen-like protein 2; CD99L2; MIC2-like protein 1; MIC2L1. |
| 研究領(lǐng)域 | 細(xì)胞生物 淋巴細(xì)胞 t-淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD99L2: 81-187/262 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May function as a homophilic adhesion molecule. Functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. Does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis. Subcellular Location: Cell membrane. Cell junction. Tissue Specificity: Expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression seen in thymus. Expressed in E18 uterus and placenta. Post-translational modifications: O-glycosylated. Similarity: Belongs to the CD99 family. SWISS: Q8TCZ2 Gene ID: 83692 Database links: Entrez Gene: 83692 Human Omim: 300846 Human SwissProt: Q8TCZ2 Human Unigene: 522805 Human |
| 產(chǎn)品圖片 |
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- CD99L2 (bs-13742R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 28 kD
Tissue/cell: human gastric cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD99L2 Polyclonal Antibody, Unconjugated(bs-13742R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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