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DARS2 Rabbit pAb (bs-14198R)  
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產品編號 bs-14198R
英文名稱 DARS2 Rabbit pAb
中文名稱 天門冬氨酰tRNA連接酶2抗體
別    名 Aspartate tRNA ligase 2 mitochondrial; Aspartate tRNA ligase; Aspartate tRNA ligase mitochondrial; Aspartyl tRNA synthetase 2(mitochondrial); Aspartyl tRNA synthetase mitochondrial; AspRS; FLJ10514; SYDM_HUMAN; LBSL; MT ASPRS; RP3 383J4.2.  
研究領域 細胞生物  信號轉導  新陳代謝  線粒體  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat (predicted: Mouse,Cow)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 68 kDa
檢測分子量
細胞定位 細胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DARS2: 551-645/645 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009]

Function:
Aspartyl-tRNA synthetase (DARS) charges its cognate tRNA with aspartate during protein biosynthesis. Mammalian synthetases differ from those of prokaryotes and lower eukaryotes in that they associate as multienzyme complexes. Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation.

Subcellular Location:
Mitochondrion matrix.

Similarity:
Belongs to the class-II aminoacyl-tRNA synthetase family.

SWISS:
Q6PI48

Gene ID:
55157

Database links:

Entrez Gene: 55157 Human

Entrez Gene: 538772 Cow

Entrez Gene: 226539 Mouse

Entrez Gene: 304919 Rat

GenBank: NM_018122.3 Human

Omim: 610956 Human

SwissProt: A6QPU5 Cow

SwissProt: Q6PI48 Human

SwissProt: Q8BIP0 Mouse

SwissProt: Q3KRD0 Rat



產品圖片
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-DARS2 Polyclonal Antibody, Unconjugated(bs-14198R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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