| 產(chǎn)品編號 | bs-18546R |
| 英文名稱 | macro H2A.1 Rabbit pAb |
| 中文名稱 | 異型組蛋白H2A.1抗體 |
| 別 名 | Core histone macro h2a.1; Core histone macro-H2A.1; H2A histone family member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2afy; H2AY_HUMAN; Histone H2A.Y; Histone macroH2A1; Histone macroH2A1.1; Histone macroH2A1.2; Macroh2a1; MACROH2A1.1; MacroH2A1.2; Medulloblastoma antigen MU MB 50.205; Medulloblastoma antigen MU-MB-50.205; mH2a; mH2A1. |
| 研究領(lǐng)域 | 細(xì)胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Pig,Sheep,Cow,Chicken,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human macro H2A.1: 1-100/372 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Subcellular Location: Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. Tissue Specificity: Ubiquitous. Post-translational modifications: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin. Similarity: Contains 1 histone H2A domain. Contains 1 Macro domain. SWISS: O75367 Gene ID: 9555 Database links: Entrez Gene: 9555 Human Entrez Gene: 26914 Mouse Omim: 610054 Human SwissProt: O75367 Human SwissProt: Q9QZQ8 Mouse Unigene: 420272 Human Unigene: 599225 Human Unigene: 283802 Mouse Unigene: 478369 Mouse Unigene: 11098 Rat |
