| 產(chǎn)品編號 | bs-18567R |
| 英文名稱 | Rabbit Anti-zcrb1 antibody |
| 中文名稱 | zcrb1蛋白抗體 |
| 別 名 | MADP 1; MADP1; RBM36; SNRNP31; U11/U12 small nuclear ribonucleoprotein 31 kDa protein; U11/U12 snRNP 31 kDa protein; U11/U12 snRNP 31K; U11/U12-31K; ZCCHC19; Zcrb1; ZCRB1_HUMAN; zinc finger CCHC-type and RNA binding motif 1; Zinc finger CCHC-type and RNA-binding motif-containing protein 1. |
| 研究領(lǐng)域 | 細胞生物 免疫學 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human zcrb1: 1-100/217 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq, Jul 2008] Subunit: Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Subcellular Location: Nucleus Similarity: Contains 1 CCHC-type zinc finger. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q8TBF4 Gene ID: 85437 Database links: Entrez Gene: 85437 Human Entrez Gene: 67197 Mouse Omim: 610750 Human SwissProt: Q8TBF4 Human SwissProt: Q9CZ96 Mouse Unigene: 496279 Human Unigene: 293181 Mouse Unigene: 136969 Rat |
| 產(chǎn)品圖片 |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- zcrb1 (bs-18567R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (zcrb1) Polyclonal Antibody, Unconjugated (bs-18567R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
