產(chǎn)品編號 | bs-12678R |
英文名稱 | Rabbit Anti-TMX3 antibody |
中文名稱 | 硫氧還蛋白相關(guān)跨膜蛋白3抗體 |
別 名 | KIAA1830; PDIA13; Protein disulfide isomerase family A, member 13; Protein disulfide-isomerase TMX3; Thioredoxin domain containing 10; Thioredoxin domain-containing protein 10; Thioredoxin-related transmembrane protein 3; TMX3; TMX3_HUMAN; TXNDC10. |
研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 跨膜蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Sheep,Dog) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 49kDa |
細(xì)胞定位 | 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TMX3: 351-450/454 <Cytoplasmic> |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 | |
產(chǎn)品圖片 |
Sample:
Lung (Mouse) Lysate at 40 ug
U251 (Human) Lysate at 40 ug
Primary: Anti-TMX3 (bs-12678R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMX3) Polyclonal Antibody, Unconjugated (bs-12678R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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