| 產(chǎn)品編號 | bs-7140R |
| 英文名稱 | Rabbit Anti-Ricin A chain antibody |
| 中文名稱 | 蓖麻毒素A鏈抗體 |
| 別 名 | Ricin toxin A chain; Ricin precursor; Ricin; rRNA N glycosidase; RT A; RTA; RICI_RICCO. |
| 產(chǎn)品類型 | 植物抗體 |
| 研究領(lǐng)域 | 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Ricinus communis) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30kDa |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Ricin A chain: 201-300/576 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ricin chain A (RTA) is a ribosome inactivating enzyme with a molecular weight of 32 kDa. RTA is normally linked by a disulfide bond to the galactose/N acetylgalactosamine binding lectin (34 kDa), also called the B chain or RTB. Together with RTB, RTA constitutes the heterodimeric cytotoxin Ricin (RCA 60) from Ricinus communis. This ricin, which is a type 2 RIP (ribosome inactivating protein) is among the most potent cytotoxins in nature. Ricin is highly toxic to animal cells and to a lesser extent to plant cells. Function: Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity). Subunit: In the N-terminal section; belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily.CuratedContains 2 ricin B-type lectin domains. SWISS: P02879 Gene ID: 8287993 Database links: Entrez Gene: 8287993 Ricinus communis |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
