| 產(chǎn)品編號 | bs-23018R |
| 英文名稱 | SLC22A17 Rabbit pAb |
| 中文名稱 | 可溶性載質(zhì)轉運蛋白22A17抗體 |
| 別 名 | 24p3 receptor; 24p3R; SLC22A17; hBOIT; BOCT; BOIT; Brain type organic cation transporter; Brain-type organic cation transporter; Neutrophil gelatinase associated lipocalin receptor; Neutrophil gelatinase-associated lipocalin receptor; Lipocalin-2 receptor; NGAL receptor; NgalR; NGALR; NGALR2; NGALR3; OTTHUMP00000082690; OTTHUMP00000082691; S22AH_HUMAN; Slc22a17; Potent brain type organic ion transporter; Solute carrier family 22 member 17; Solute carrier family 22 organic cation transporter member 17. |
| 研究領域 | 腫瘤 免疫學 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC22A17: 211-300/538 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SLC22A17 (solute carrier family 22 nember 17; organic cation transportor,).may act as a brain-specific organic ion transporter. The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. Function: Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis (By similarity). Subcellular Location: Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note=Upon LCN2-binding, it is internalized. Tissue Specificity: Expressed in brain. Similarity: Belongs to the major facilitator superfamily. Organic cation transporter family. SWISS: Q8WUG5 Gene ID: 51310 Database links: Entrez Gene: 51310 Human Omim: 611461 Human SwissProt: Q8WUG5 Human Unigene: 373498 Human Unigene: 7312 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Lane 3: U87MG (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Lane 5: Siha (Human) Cell Lysate at 30 ug
Primary:
Anti-SLC22A17 (bs-23018R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (bs-23018R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (bs-23018R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (bs-23018R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
