| 產(chǎn)品編號 | bsm-33142M |
| 英文名稱 | eIF4A1 Mouse mAb |
| 中文名稱 | eIF4A1蛋白單克隆抗體 |
| 別 名 | ATP-dependent RNA helicase eIF4A-1; DDX2; DDX2A; eIF 4A I; eIF-4A-I; EIF4A; eIF4A I; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; Eukaryotic translation initiation factor 4A1; IF4A1_HUMAN. |
| 研究領域 | 細胞生物 表觀遺傳學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 1B8 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human eIF4A1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5’-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5’ cap, eIF4A(I,II) are bidirectional RNA helicases, and eIF4G(I,II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2. Function: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Subunit: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with human cytomegalovirus/HHV-5 protein UL69. Similarity: Belongs to the DEAD box helicase family. eIF4A subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: P60842 Gene ID: 1973 Database links: Entrez Gene: 1973 Human Entrez Gene: 13681 Mouse Omim: 602641 Human SwissProt: P60842 Human SwissProt: P60843 Mouse Unigene: 129673 Human Unigene: 279821 Mouse Unigene: 371557 Mouse Unigene: 91523 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Raw264.7 cell lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Human HepG2 cell lysates
Lane 4: Human K562 cell lysates
Lane 5: Human PANC-1 cell lysates
Primary: Anti-eIF4A1 (bsm-33142M) at 1/1000 dilution
Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific
Predicted band size: 46 kDa
Observed band size: 46 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
