| 產(chǎn)品編號 | bsm-33088M |
| 英文名稱 | CD23 Mouse mAb |
| 中文名稱 | CD23/FcεRII單克隆抗體 |
| 別 名 | Fc EpsilonR II; FcεRII; Blast 2; Blast2; C type lectin domain family 4 member J; C-type lectin domain family 4, member J; CD 23; CD 23A; CD23 antigen; CD23A; CLEC 4J; CLEC4J; Fc epsilon RII; Fc fragment of IgE low affinity II receptor for; Fc fragment of IgE, low affinity II, receptor for(CD23); Fc of IgE, low affinity II, receptor for(CD23); Fc receptor IgE low affinity II alpha polypeptide; Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a; FCE 2; FCE2; FCER 2; FCER2; FCER2A; IgE binding factor; IgE receptor lymphocyte; IgE-binding factor; IGEBF; Immunoglobulin E binding factor; Immunoglobulin E receptor, low affinity II; Low affinity immunoglobulin epsilon Fc receptor; Low affinity immunoglobulin epsilon Fc receptor membrane bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; Ly-42; Ly42; Lymphocyte antigen CD23; Lymphocyte IgE receptor; MGC93219. |
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Specific References (1) | bsm-33088M has been referenced in 1 publications.
[IF=6.922] Maoyu Ye. et al. Long-Term Exposure to Sulfur Dioxide Before Sensitization Decreased the Production of Specific IgE in HDM-Sensitized Allergic Rhinitis Mice. J INFLAMM RES. 2022; 15: 2477–2490 IF ; Mouse.
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| 研究領域 | 細胞生物 免疫學 b-淋巴細胞 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 12G2 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CD23 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets and EBV transformed B lymphoblasts. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia. CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils. CD23 is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a “membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro. Function: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). Subcellular Location: Cell membrane; Single-pass type II membrane protein. Secreted. Note=Also exists as a soluble excreted form. Post-translational modifications: N- and O-glycosylated. Similarity: Contains 1 C-type lectin domain. SWISS: P06734 Gene ID: 2208 Database links: Entrez Gene: 2208 Human Entrez Gene: 14128 Mouse Omim: 151445 Human SwissProt: P06734 Human SwissProt: P20693 Mouse Unigene: 465778 Human Unigene: 1233 Mouse CD23是一種II型跨膜糖蛋白，是存在于B細胞表面的IgE受體。在慢性B淋巴細胞白血?。–LL）和小B細胞淋巴瘤（SLL）為陽性，套細胞淋巴瘤和小核裂淋巴細胞淋巴瘤陰性。也表達于外周血B細胞的某些亞型、B淋巴細胞和EB病毒轉(zhuǎn)染的B淋巴瘤細胞系。CD23是慢性B淋巴細胞白血病，小B細胞淋巴瘤診斷和鑒別診斷有用的標記物。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
The Daudi(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD23 antibody (bsm-33088M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
