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Cyclooxygenase 2 Mouse mAb (bsm-33171M)  
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產(chǎn)品編號 bsm-33171M
英文名稱 Cyclooxygenase 2 Mouse mAb
中文名稱 環(huán)氧合酶2/前列腺素內(nèi)過氧化物合成酶2單克隆抗體
別    名 PTGS2; COX 2; COX2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN.  
研究領(lǐng)域 腫瘤  心血管  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  合成與降解  新陳代謝  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 5E5
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 65 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Cyclooxygenase 2 
亞    型 IgG
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Function:
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

Subunit:
Homodimer.

Subcellular Location:
Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Post-translational modifications:
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.

Similarity:
Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain.

SWISS:
P35354

Gene ID:
5743

Database links:

Entrez Gene: 5743 Human

Entrez Gene: 19225 Mouse

Entrez Gene: 29527 Rat

Omim: 600262 Human

SwissProt: P35354 Human

SwissProt: Q05769 Mouse

SwissProt: P35355 Rat

Unigene: 196384 Human

Unigene: 292547 Mouse

Unigene: 44369 Rat



合成與降解(Synthesis and Degradation)
COX-2 前列腺素氧化環(huán)化酶-2是前列腺素合成的關(guān)鍵酶之一,為誘導(dǎo)性酶,他參與細(xì)胞對生長因子、腫瘤促進(jìn)因子和細(xì)胞因子的反應(yīng),這些因子均能誘導(dǎo)COX2表達(dá),它也參與前列腺素的合成。
COX2在80-90%的人結(jié)腸腺癌中表達(dá)顯著增高,而COX1表達(dá)卻不變。
產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Cyclooxygenase 2 monoclonal antibody, unconjugated (bsm-33171M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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