| 產(chǎn)品編號 | bsm-33185M |
| 英文名稱 | EBFP Mouse mAb |
| 中文名稱 | 增強型藍色熒光蛋白單克隆抗體 |
| 別 名 | Blue Fluorescent Protein; Enhanced Blue Fluorescent Protein. 藍色熒光蛋白的工程變體蛋白 |
| 產(chǎn)品類型 | 標簽抗體 |
| 研究領域 | 細胞生物 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 7B12 |
| 交叉反應 | Recombinant protein |
| 產(chǎn)品應用 | WB=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant EBFP protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: EBFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33185P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti- EBFP-Tag (bsm-33185M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
