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BiossECL Plus WB Substrate (C05-07004)  
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訂購(gòu)QQ:  400-901-9800
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說(shuō)明書(shū): 下載
12.5ml×2/280.00元
50ml×2/880.00元
250ml×2/3200.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) C05-07004
英文名稱 BiossECL Plus WB Substrate
中文名稱 BiossECL超敏發(fā)光液
別    名 ECL Western Blotting Substrate; BiossECL Plus Western Blotting Substrate Kit; ECL Western Blotting Substrate Kit; Ultrasensitive ECL Kit; BiossECL Plus; Western Blotting Luminol Reagent;   極超敏ECL化學(xué)發(fā)光試劑盒; UltraSignal 超敏ECL化學(xué)發(fā)光底物;UltraSignal 超敏ECL化學(xué)發(fā)光液; ECL Plus超敏發(fā)光液; ECL超敏發(fā)光液; ECL超敏發(fā)光底物; ECL發(fā)光液; ECL Plus級(jí)發(fā)光液; 超敏化學(xué)發(fā)光檢測(cè)試劑盒; Western螢光檢測(cè)試劑盒; 魯米諾化學(xué)發(fā)光試劑; 增強(qiáng)型ECL 化學(xué)發(fā)光檢測(cè)試劑盒(即用型);
Specific References  (16)     |     C05-07004 has been referenced in 16 publications.
[IF=17.694] Guo, Yuxin. et al. In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses. NAT COMMUN. 2022 Nov;13(1):1-20  
[IF=10.317] Zhuo Wang. et al. Targeted galectin-7 inhibition with ultrasound microbubble targeted gene therapy as a sole therapy to prevent acute rejection following heart transplantation in a Rodent model. Biomaterials. 2020 Dec;263:120366  
[IF=6.792] Li Z et al. Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats. Environ Pollut . 2020 Oct;265(Pt A):115025.  WB ;  
[IF=4.872] Junyu Hou. et al. Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats. Ecotox Environ Safe. 2021 Apr;212:112012  other ;  
[IF=3.848] Zuo Qiang. et al. Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway. Acta Bioch Bioph Sin. 2021 Sep;:  WB ;  human .  
[IF=3.654] Mengying Zhang. et al. Preparation of Black pepper (Piper nigrum L.) essential oil nanoparticles and its antitumor activity on triple negative breast cancer in vitro. J FOOD BIOCHEM. 2022 Sep;:e14406  Other ;  Other.  
[IF=3.647] Zhang C et al. Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in triple negative breast cancer cellsLife Sci.2020 Dec 10;266:118886.  WB ;  
[IF=3.34] Zhang, Yi-Dan. et al. Role of synaptotagmin 13 (SYT13) in promoting breast cancer and signaling pathways. CLIN TRANSL ONCOL. 2023 Jan;:1-12  
[IF=3.298] Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;():  
[IF=3.056] Shengda Li. et al. Polystyrene microplastics induce blood–testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats. 2021 Apr 25  Other ;  
[IF=2.952] Guanglei Han. et al. Salvianolic acid?B acts against non?small cell lung cancer A549 cells via inactivation of the MAPK and Smad2/3 signaling pathways. Mol Med Rep. 2022 May;25(5):1-11  Other ;  Other.  
[IF=2.903] Fangming Guo. et al. Shenfu Administration Improves Cardiac Fibrosis in Rats With Myocardial Ischemia-Reperfusion Through Adenosine A2a Receptor Activation:. Hum Exp Toxicol. 2022;():  Other ;  Other.  
[IF=2.745] Zhonghua An. et al. Chamomile Essential Oil: chemical constituents and antitumor activity in MDA-MB-231 cells through PI3K/Akt/mTOR signaling pathway. CHEM BIODIVERS. 2023 Mar;:e202200523  
[IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292  Other ;  Other.  
[IF=2.315] Lin Chen. et al. Preparation, structural characterization and neuroprotective effects of polysaccharides from the pericarp of Zanthoxylum bungeanum Maxim against H2O2-induced oxidative damage in PC12 cells. S Afr J Bot. 2021 Nov;142:165  WB ;  Rat.  
[IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
保存條件 Store at 2-8℃. Protect from light for one year.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 簡(jiǎn)介
BiossECL超敏發(fā)光液直接或間接檢測(cè)與辣根過(guò)氧化物酶HRP關(guān)聯(lián)的蛋白或核酸底物。這一獨(dú)特的發(fā)光底物系統(tǒng)是目前最靈敏的商業(yè)化熒光ECL檢測(cè)試劑,具有極高靈敏度和高信噪比,可檢測(cè)出10-100 fg的微量抗原;發(fā)光迅速,熒光可使X 感光膠片感光達(dá)12小時(shí)以上,特別適用于痕量蛋白或核酸檢測(cè)。同時(shí)該試劑允許使用更高的抗體稀釋倍數(shù)(1:2000-1:10000),極其節(jié)省抗體。

試劑盒內(nèi)容
25ml規(guī)格:A液12.5ml + B液12.5ml
100ml規(guī)格:A液50ml + B液50ml
500ml規(guī)格:A液250ml + B液250ml

顯色步驟
1、執(zhí)行常規(guī)SDS-PAGE、轉(zhuǎn)膜和Western Blot封閉、一抗孵育、二抗孵育操作。
2、二抗適用HRP標(biāo)記二抗,適用恰當(dāng)?shù)南♂尡壤?br /> 3、制備ECL工作液:A液和B液等體積的混合,例如1ml A液和1ml B液混合,混勻后即可使用。用量以充分覆蓋印跡膜為基準(zhǔn),每10 cm2膜需要大約1 ml工作液。
4、將印跡膜有蛋白的一面朝上平整的鋪在一張平板上,加上配好的發(fā)光底物工作液。
5、將工作液均勻滴加在印跡膜上,孵育1-5min。可將一潔凈的保鮮膜或透明的玻璃紙平整的鋪在孵育有工作液的印跡膜上,輕輕趕出其間的氣泡。
6、在暗室中取一張X膠片小心置于膜上,曝光5秒至1分鐘,立即顯影定影。
7、根據(jù)其發(fā)光的強(qiáng)度,縮短或延長(zhǎng)X片的曝光時(shí)間(對(duì)微弱信號(hào),曝光時(shí)間可延長(zhǎng)至數(shù)小時(shí)),或者曝光一系列不同的時(shí)間后再顯影定影挑選一張滿意的。也可用合適的照相器材直接記錄蛋白膜的化學(xué)發(fā)光圖像。

安全性:
無(wú)特殊毒性,按普通化學(xué)品處理。如果不慎與眼、皮膚和衣物接觸,請(qǐng)立刻用大量清水沖洗。

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