產(chǎn)品編號(hào) | C05-06002 |
英文名稱 | Western Blocking Buffer (5%BSA) |
中文名稱 | WB封閉液(5%BSA) |
別 名 | WB Blocking Buffer (BSA); WB Blocking Buffer (5%BSA); 5% BSA Blocking Buffer; Western封閉液(BSA); 5%BSA封閉液; |
Specific References (4) | C05-06002 has been referenced in 4 publications.
[IF=9.221] Cuixia Guo. et al. Thickness and fluorescence-based dual-encoded suspension array and corresponding decoding system for multiplexed detection. SENSOR ACTUAT B-CHEM. 2023 Apr;:133793
[IF=3.423] Qi-Lin Lu. et al. Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy. MOL MED REP. 2022 Sep;26(3):1-9
[IF=2.571] Lu Z et al. The role of N 6-methyladenosine RNA methylation in the heat stress response of sheep (Ovis aries). Cell Stress Chaperones. 2019 Jan 30. IHC-P ;
[IF=2.379] Zheng X et al. Protosappanin B Exerts Anti-tumor Effects on Colon Cancer Cells via Inhibiting GOLPH3 Expression:Integr Cancer Ther.Jan-Dec 2020;19:1534735420972477. WB ;
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保存條件 | -20℃保存一年,短期保存可置于2-8℃。. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品介紹 |
簡(jiǎn)介 WB封閉液(Western Blocking Buffer)可用于WB時(shí)轉(zhuǎn)移了蛋白樣品的NC膜或PVDF膜非特異性結(jié)合位點(diǎn)的封閉。封閉后,可以減少后續(xù)的一抗或二抗與膜的非特異性結(jié)合,降低背景著色,增強(qiáng)信噪比。 本產(chǎn)品組分為0.01M TBS(pH7.4),含5%BSA。 使用說(shuō)明: 完成轉(zhuǎn)膜后,用WB洗滌液洗滌蛋白膜1-2分鐘。加入WB封閉液,封閉60分鐘,然后進(jìn)行一抗孵育等后續(xù)操作。 注意事項(xiàng): 1.本產(chǎn)品屬蛋白制劑,室溫放置過(guò)久易導(dǎo)致蛋白的降解和活性喪失。 2.反復(fù)凍融亦會(huì)導(dǎo)致蛋白活性的喪失。 3.本產(chǎn)品含防腐劑,為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。 4.通常在室溫封閉60分鐘即可。如果對(duì)于一些抗體,背景非常高,可以在4℃封閉過(guò)夜。 |