產(chǎn)品編號(hào) | C05-07003 |
英文名稱 | 10×TBST, pH=7.4 |
中文名稱 | 10×TBST緩沖液 pH=7.4 |
別 名 | Tween20/TBS solution (10×TBST,pH7.4); WB Washing Buffer, 10×; Western Blot Wash Buffer, 10×; 10×Western Blot Wash Buffer; 10*TBST; TBS-T; WB洗液(10X); Tween20/TBS溶液(10×TBST,pH7.4); Western洗滌液(10X); Western洗滌液 (10×); WB洗液(10×); Western 洗滌液(10×); Western洗滌液(10×); 10×TBST緩沖液; Western洗滌液(10×) |
Specific References (3) | C05-07003 has been referenced in 3 publications.
[IF=5.572] Jiayuan Luo. et al. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas. FOOD CHEM TOXICOL. 2023 May;175:113724
[IF=3.77] Jiachao Shen. et al. Cyclosporin A improves the hyperosmotic response in an experimental dry eye model by inhibiting the HMGB1/TLR4/NF-κB signaling pathway. EXP EYE RES. 2023 Apr;229:109418
[IF=1.871] Bi H et al. Interleukin?8 promotes cell migration via CXCR1 and CXCR2 in liver cancer. ONCOLOGY LETTERS.2019 18: 4176-4184. WB ;
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保存條件 | Store at 2-8℃ for one year. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品介紹 |
簡(jiǎn)介 本產(chǎn)品可以用于WB時(shí)一抗或二抗孵育后的洗滌。適當(dāng)?shù)南礈炜山档捅尘?,增?qiáng)信噪比。 使用說明: 取適量本產(chǎn)品,用去離子水10倍稀釋。在一抗或二抗孵育結(jié)束后,加入10ml的WB洗滌液,使之能蓋住蛋白膜,在搖床上緩慢搖動(dòng)洗滌3-5分鐘后,吸盡洗滌液,再加入新的WB洗滌液。共洗滌3次,每次3-5分鐘。然后進(jìn)行后續(xù)的操作。如果按照上述洗滌條件,WB的染色結(jié)果背景仍然較高,可以適當(dāng)延長(zhǎng)洗滌時(shí)間,并增加洗滌次數(shù)。通常,延長(zhǎng)洗滌時(shí)間或增加洗滌次數(shù)不會(huì)對(duì)WB的結(jié)果產(chǎn)生負(fù)面影響。 注意事項(xiàng): 1.本產(chǎn)品經(jīng)過濾除菌處理,使用過程中應(yīng)盡量避免細(xì)菌污染。一旦啟用后建議在2周內(nèi)用完。 2.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。 |