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GPX3 Rabbit pAb (bs-22009R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-22009R
英文名稱 GPX3 Rabbit pAb
中文名稱 谷胱甘肽過氧化酶3
別    名 Extracellular glutathione peroxidase; Glutathione peroxidase 3(plasma); Glutathione peroxidase 3; GPX 3; GPx P; GPx-3; GPx-P; GPX3; GPX3_HUMAN; GPXP; GSHPx 3; GSHPx P; GSHPx-3; GSHPx-P; Plasma glutathione peroxidase 3; Plasma glutathione peroxidase.  
Specific References  (2)     |     bs-22009R has been referenced in 2 publications.
[IF=5.719] Giovanna Chianese. et al. ROS-Generating Hyaluronic Acid-Modified Zirconium Dioxide-Acetylacetonate Nanoparticles as a Theranostic Platform for the Treatment of Osteosarcoma. NANOMATERIALS-BASEL. 2023 Jan;13(1):54  IF ;  Human.  
[IF=2.141] Ting Yao. et al. Differences of the anti-oxidative capability, GPX3, and Cu/ZnSOD expression in Hu sheep testis with different size at six-month-old. ANIM BIOTECHNOL. 2023 Feb 15  IHC ;  Sheep.  
研究領(lǐng)域 細胞生物  信號轉(zhuǎn)導  新陳代謝  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Pig,Cow)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23 kDa
檢測分子量
細胞定位 分泌型蛋白 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GPX3: 1-100/226 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Glutathione peroxidases(Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organichydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPxfamily to be discovered. GPx2, also known as gastrointestinal GPx(GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract.Extracellular plasma GPx(pGPxor GPx3) is mainly expressed by the kidney from where it is released into the blood. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxylipids in low density lipoprotein orthymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx(eGPxor GPx5)isoforms, possess selenocysteine at the active site.

Function:
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Subunit:
Homotetramer.

Subcellular Location:
Secreted.

Tissue Specificity:
Secreted in plasma.

Post-translational modifications:
The N-terminus is blocked.

Similarity:
Belongs to the glutathione peroxidase family.

SWISS:
P22352

Gene ID:
2878

Database links:
UniProtKB/Swiss-Prot: P22352.2

谷胱甘肽過氧化物酶(GSH-Px)是機體內(nèi)廣泛存在的一種重要的過氧化物分解酶。硒是GSH-Px酶系的組成成分,它能催化GSH變?yōu)镚SSG,使有毒的過氧化物還原成無毒的羥基化合物,同時促進H2O2的分解,從而保護細胞膜的結(jié)構(gòu)及功能不受過氧化物的干擾及損害。GSH-Px的活性中心是硒半胱氨酸,其活力大小可以反映機體硒水平。GSH-Px酶系主要包括4種不同的GSH-Px,分別為胞漿GSH-Px、血漿GSH-Px、磷脂氫過氧化物GSH-Px及胃腸道專屬性GSH-Px。胞漿GSH-Px由4個相同的分子量大小為22kDa的亞基構(gòu)成四聚體,每個亞基含有1個分子硒半胱氨酸,廣泛存在于機體內(nèi)各個組織,以肝臟紅細胞為最多。它的生理功能主要是催化GSH參與過氧化反應,清除在細胞呼吸代謝過程中產(chǎn)生的過氧化物和羥自由基,從而減輕細胞膜多不飽和脂肪酸的過氧化作用
產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPX3) Polyclonal Antibody, Unconjugated (bs-22009R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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