| 產(chǎn)品編號 | bsk11005 |
| 英文名稱 | Human IL-5 ELISA Kit |
| 中文名稱 | 人白細(xì)胞介素5酶聯(lián)免疫試劑盒 |
| 別 名 | IL5; EDF; IL-5; TRF; interleukin 5 |
| 克 隆 號 | |
| 種 屬 | Human |
| 線性范圍 | 1.60 - 100 pg/mL |
| 應(yīng)用范圍 | S/P/CC |
| 檢測限 | 1 pg/mL |
| 適用樣品基質(zhì) | cell culture supernates, serum, and plasma. |
| 保存條件 | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.). |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Interleukin-5(IL-5)is a secreted glycoprotein that belongs to the α-helical group of cytokines. Unlike other family members, it is present as a covalently linked antiparallel dimer. The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross-reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow. The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 Rα) and a shared Signaltransducing subunit, βc. IL-5 Rα first binds IL-5 at low affinity, then associates with preformed βc dimers, forming a high-affinity receptor. IL-5 also binds proteoglycans, potentially enhancing its activity. Soluble forms of IL-5 Rα antagonize IL-5 and can be found in vivo. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release .
白介素 (IL) 5是一種主要在特應(yīng)性疾病中起致病作用的細(xì)胞因子 ,又名Ｂ細(xì)胞分化因子 - μ、Ｂ細(xì)胞生長因子 2、Ｔ細(xì)胞替代因子、嗜酸粒細(xì)胞集落刺激因子 1、嗜酸粒細(xì)胞分化因子等。它調(diào)控著嗜酸粒細(xì)胞的產(chǎn)生、活化和分布 ,對Ｂ細(xì)胞、Ｔ細(xì)胞、嗜酸粒細(xì)胞、嗜堿粒細(xì)胞具有多種生物學(xué)功能。Azuma等于1986年克隆成功，是一種具有 白介素5多種生物學(xué)功能的淋巴因子。根據(jù)IL-5的作用，它被命名為T細(xì)胞替代因子（TRF）、B細(xì)胞生長因子Ⅱ（BCGFⅡ），嗜酸性粒細(xì)胞分化因子（EDF）和IgA增強因子。 |
