| 產(chǎn)品編號 | bs-48015P |
| 英文名稱 | Recombinant human BMP-2 protein (Active, HEK293) |
| 中文名稱 | 重組人骨形態(tài)發(fā)生蛋白-2 (活性的, Tag free) |
| 別 名 | rhBMP-2; Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN; Recombinant human BMP-2 protein (Active, HEK293) 人骨形態(tài)發(fā)生蛋白-2重組蛋白; 重組人骨形態(tài)發(fā)生蛋白-2; 人重組骨形態(tài)發(fā)生蛋白-2; 重組人BMP2; |
| 檢測分子量 | 30-38 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 283-396/396 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內(nèi)毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統(tǒng) | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Bone morphogenetic protein 2 (BMP-2) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-2 is expressed abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. BMP-2 plays an important role in inducing bone and cartilage formation. Mature human BMP-2 is usually a homodimer consisting of two monomers of 114 amino acids. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif. This recombinant human BMP-2 is produced by human cells.(Tag free) Biological activity: The activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P12643 Gene ID: 650 |
| 產(chǎn)品圖片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
