| 產(chǎn)品編號(hào) | bsm-51487M |
| 英文名稱(chēng) | CAMKK2 Mouse mAb |
| 中文名稱(chēng) | 鈣調(diào)蛋白激酶激酶β單克隆抗體 |
| 別 名 | Calcium/calmodulin dependent protein kinase beta; Calcium/calmodulin dependent protein kinase kinase 2; Calcium/calmodulin dependent protein kinase kinase beta; CaM kinase kinase beta; CaM KK beta; CAMKK 2; CAMKK; CaMKK beta; CAMKK beta protein; CAMKKB. |
| 研究領(lǐng)域 | 腫瘤 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Monoclonal |
| 克 隆 號(hào) | C5F12 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CAMKK2. |
| 亞 型 | IgG1, k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases. [provided by RefSeq, Jul 2008]. Function: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals. Seems to be involved in hippocampal activation of CREB1. May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching. Subunit: Interacts with calmodulin. Subcellular Location: Nucleus. Cytoplasm. Cell projection. Note=Predominantly nuclear in unstimulated cells. Found in the cytoplasm and neurites after forskolin induction. Tissue Specificity: Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung. Post-translational modifications: Autophosphorylated and phosphorylated by PKA. Each isoform may show a different pattern of phosphorylation. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. SWISS: Q96RR4 Gene ID: 10645 Database links: Entrez Gene: 10645 Human SwissProt: Q96RR4 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Primary: Anti-CAMKK2 (bsm-51487M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 58 kD
Sample:
Lane 1: Jurkat cell lysates
Primary: Anti-CAMKK2 (bsm-51487M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 58 kD
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