| 產(chǎn)品編號(hào) | bs-34202R |
| 英文名稱 | LDHA Rabbit pAb |
| 中文名稱 | 乳酸脫氫酶抗體 |
| 別 名 | Lactate Dehydrogenase; Lactate Dehydrogenase Isoenzyme V; Lactate Dehydrogenase isozyme H4; L lactate dehydrogenase A chain; Lactate dehydrogenase A; Lactate dehydrogenase A chain; LDH A; LDH heart subunit; LDH M; LDHM; LDH muscle subunit; LDH1; LDHA; PIG 19; PIG19; Proliferation inducing gene 19 protein; TRG 5; TRG5; LDHA_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LDHA |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]. Subunit: Homotetramer. Subcellular Location: Cytoplasm. Post-translational modifications: ISGylated. DISEASE: Glycogen storage disease 11 (GSD11) [MIM:612933]: A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the LDH/MDH superfamily. LDH family. SWISS: P00338 Gene ID: 3939 Database links: Entrez Gene: 3939 Human Entrez Gene: 16828 Mouse Omim: 150000 Human SwissProt: P00338 Human SwissProt: P06151 Mouse Unigene: 2795 Human Unigene: 29324 Mouse Unigene: 107896 Rat |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with LDHA polyclonal antibody, unconjugated (bs-34202R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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