| 產品編號 | bsm-34219M |
| 英文名稱 | BHLHA15 Mouse mAb |
| 中文名稱 | BHLHA15單克隆抗體 |
| 別 名 | BHA15_HUMAN; Class A basic helix-loop-helix protein 15; bHLHa15; Class B basic helix-loop-helix protein 8(bHLHb8); Muscle, intestine and stomach expression 1(MIST-1); BHLHB8; MIST1; basic helix-loop-helix family member a15; |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 3C4 |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig) |
| 產品應用 | WB=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | 27 |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinded human BHLHA15 protein: 1-189/189 |
| 亞 型 | IgG2a |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in several processes, including cellular response to glucose starvation; endoplasmic reticulum unfolded protein response; and negative regulation of myotube differentiation. Predicted to act upstream of or within several processes, including glucose homeostasis; intracellular distribution of mitochondria; and mitochondrial calcium ion transmembrane transport. Predicted to be part of chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022] Function: Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Expressed in brain, liver, spleen and skeletal muscle. SWISS: Q7RTS1 Gene ID: 168620 Database links: Entrez Gene: 168620 Human SwissProt: Q7RTS1 Human |
| 產品圖片 |
Sample:
Lane 1: Human K562 cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human 293T cell lysates
Primary: Anti-MIST1 (bsm-34219M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 27 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
