| 產(chǎn)品編號(hào) | bsm-34121M |
| 英文名稱 | Mart-1 Mouse mAb |
| 中文名稱 | 黑色素瘤相關(guān)抗原單克隆抗體 |
| 別 名 | MAR1_HUMAN; Melanoma antigen recognized by T-cells 1; MLANA; MART1; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A; |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | F4D11 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mart-1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Melan A, a product of the MART-1 gene, is a melanocyte differentiation marker recognized by autologous cytotoxic T lymphocytes. Other melanoma-associated markers recognized by autologous cytotoxic T cells are reported to include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. The analysis of these different molecules and their expression in individual melanomas may be of help in the study of their particular molecular roles in melanocyte differentiation and tumorigenesis. SWISS: Q16655 Gene ID: 2315 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Mart-1 monoclonal antibody, unconjugated (bsm-34121M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mart-1 Monoclonal Antibody, Unconjugated (bsm-34121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mart-1 Monoclonal Antibody, Unconjugated (bsm-34121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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