| 產(chǎn)品編號 | bs-41611R |
| 英文名稱 | VEGFR1 Rabbit pAb |
| 中文名稱 | 血管內皮生長因子受體1抗體 |
| 別 名 | Vascular endothelial growth factor receptor 1; VEGF-R1; VEGFR-1; VEGF Receptor 1; FLT-1; vascular permeability factor receptor; vascular endothelial growth factor receptor; vascular endothelial growth factor receptor-1; fms-related tyrosine kinase 1; vascular endothelial growth factor/vascular permeability factor receptor; AI323757; FLT; FLT1; sFlt1; VGFR1_HUMAN; VGFR1_MOUSE. |
| 研究領域 | 腫瘤 細胞生物 免疫學 發(fā)育生物學 神經(jīng)生物學 生長因子和激素 激酶和磷酸酶 細胞膜受體 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 147 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human VEGFR1 protein: 27-687/687 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes. SWISS: P17948 Gene ID: 2321 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant human VEGFR1 protein, C-His (HEK293)( bs-41611P)
Primary: Anti-VEGFR1 (bs-41611R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 140 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VEGFR1) Polyclonal Antibody, Unconjugated (bs-41611R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
