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CD8A Mouse mAb (bsm-34251M)  
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50ul/1180.00元
100ul/1980.00元
大包裝/詢價

產(chǎn)品編號 bsm-34251M
英文名稱 CD8A Mouse mAb
中文名稱 CD8A單克隆抗體
別    名 CD8 antigen, alpha polypeptide(p32); CD8a; CD8a antigen; CD8a molecule; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen.  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 IHC-P=1:500-1000,IHC-F=1:500-1000,IF=1:500-1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 27 kDa
檢測分子量
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD8A 
亞    型 IgG1, k
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]

Function:
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit:
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Most prominently found in the gastrointestinal tract, skin, lung, and brain but not in liver.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P01732

Gene ID:
925

Database links:

Entrez Gene: 925 Human

Entrez Gene: 12525 Mouse

SwissProt: P01732 Human

SwissProt: P01731 Mouse



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (bsm-34251M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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