| 產(chǎn)品編號 | bsm-43646M |
| 英文名稱 | E-Selectin Mouse mAb |
| 中文名稱 | E選擇素單克隆抗體 |
| 別 名 | CD 62E; CD62E; CD62E antigen; CD62 antigen-like family member E; E selectin; ELAM 1; ELAM; ELAM-1; ELAM1; Endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM 2; LECAM2; Leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; LYAM2_HUMAN; SELE; Selectin E; SelectinE. |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 1G1 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:100-500,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 免 疫 原 | Recombinant human E-Selectin protein |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M PBS(pH7.4). |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008] Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Subunit: Interacts with PSGL1/SELPLG through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 6 Sushi (CCP/SCR) domains. SWISS: P16581 Gene ID: 6401 Database links: Entrez Gene: 6401 Human Entrez Gene: 20339 Mouse Omim: 131210 Human SwissProt: P16581 Human SwissProt: Q00690 Mouse Unigene: 82848 Human Unigene: 5245 Mouse Unigene: 10359 Rat E選擇素(CD62E)是活化的內(nèi)皮細胞表面表達的黏附分子,是白細胞與內(nèi)皮細胞形成接觸時的啟動因子。E選擇素是表達在血管內(nèi)皮細胞表面的黏附分子。 |
| 產(chǎn)品圖片 |
100 ng rhE-Selectin-His protein (bs-43646P) per lane probed with E-Selectin monoclonal antibody respectively, unconjugated (bsm-43646M) at 1:200 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(bsm-43646M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(bsm-43646M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
