| 產品編號 | bsm-63185R |
| 英文名稱 | RAF1 Recombinant Rabbit mAb |
| 中文名稱 | 原癌基因RAF1重組兔單抗 |
| 別 名 | C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 6B6 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-1:2000,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 73 kDa |
| 檢測分子量 | 73 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human c Raf: 1-67 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. SWISS: P04049 Gene ID: 5894 Raf-1（c-Raf）是一種涉及促有絲分裂信號從細胞膜傳導到核的蛋白激酶。Raf-1被結合GTP的Ras所征募并與之結合，活化MEK-MAP激酶通路。Raf-1在哺乳動物中通過多個位點的快速高度磷酸化而被活化。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
