| 產(chǎn)品編號 | bsm-63287R |
| 英文名稱 | RPS6KA1 Recombinant Rabbit mAb |
| 中文名稱 | 絲氨酸/蘇氨酸激酶p90RSK蛋白重組兔單抗 |
| 別 名 | HU1; MAPKAPK1A p90 RSK1; p90rsk; p90RSK1; p90S6K; pp90RSK1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK1; S6K alpha 1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 5H2 |
| 交叉反應(yīng) | Human,Mouse,Rat,Pig |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 83 kDa |
| 檢測分子量 | 83 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human p90 S6K alpha 1: 1-67 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation. Interacts with ETV1/ER81 and FGFR1.Interacts with Kaposi's sarcoma-associated herpesvirus/HHV-8 protein ORF45; this interaction allows RPS6KA1 activation. Gene ID: 6195 p90RSK蛋白屬于廣泛表達的絲氨酸/蘇氨酸激酶。RSK家族包括RSK1、RSK2、RSK3和RSK4，它們的典型特征是含有兩個不同的功能性激酶結(jié)構(gòu)域和羧基端ERKs(extracellular signal-regulated kinases)結(jié)合結(jié)構(gòu)域。RSK激酶結(jié)構(gòu)域內(nèi)外的幾個位點包括Ser380、Thr359、Ser363和Thr573的磷酸化都對RSK的激酶活性激活非常重要。 RSK1-3可被MAPKs所磷酸化激活，也可以通過自身磷酸化激活，還可被一些生長因子或神經(jīng)遞質(zhì)等誘導(dǎo)激活的PI3K信號通 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
