| 產(chǎn)品編號(hào) | bsm-52390R |
| 英文名稱 | NADPH oxidase 4 Recombinant Rabbit mAb |
| 中文名稱 | NADPH氧化酶4抗體 |
| 別 名 | KOX 1; KOX; Nox 4; Nox-4; NADPH oxidase 4; RENOX; Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; Kox-1; NADPH; Nox4; NOX4_HUMAN; Renal NAD(P)H-oxidase; RENOX. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 2A4 |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human NOX4: 510-578 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response. Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. Subunit : Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with protein disulfide isomerase. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Cell membrane. Note=May localize to plasma membrane and focal adhesions. Tissue Specificity: EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. Similarity: Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain. SWISS: Q9NPH5 Gene ID: 50507 Database links: Entrez Gene: 50507 Human Entrez Gene: 50490 Mouse Omim: 605261 Human SwissProt: Q9NPH5 Human SwissProt: Q9JHI8 Mouse Unigene: 371036 Human Unigene: 31748 Mouse Unigene: 14744 Rat 還原型輔酶煙酰胺腺嘌呤二核苷酸磷酸(NADPH)oxidase 4:還原型輔酶煙胺腺嘌呤二核苷酸（Nicotinamide adenine dinucleotide reduced, NADH）位于線粒體膜內(nèi)，是細(xì)胞能量代謝所必需的輔酶，主要功能是經(jīng)電子傳遞鏈產(chǎn)生ATP。NADH在維持細(xì)胞生長(zhǎng)、分化和能量代謝中起重要的作用。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with NADPH oxidase 4 monoclonal antibody, unconjugated (bsm-52390R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NADPH oxidase 4 Monoclonal Antibody, Unconjugated(bsm-52390R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NADPH oxidase 4 Monoclonal Antibody, Unconjugated(bsm-52390R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NADPH oxidase 4 Monoclonal Antibody, Unconjugated(bsm-52390R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NADPH oxidase 4 Monoclonal Antibody, Unconjugated(bsm-52390R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NADPH oxidase 4 Monoclonal Antibody, Unconjugated(bsm-52390R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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