| 產(chǎn)品編號 | bs-11134R-Bio |
| 英文名稱 | Rabbit Anti-PCDHB15/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的原鈣粘蛋白15抗體 |
| 別 名 | PCDH beta15; PCDHB 15; PCDHB15; PCDHB-15; Protocadherin beta 15; PCDHB 22; PCDHB-22; PCDHB22; PCDBF_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞粘附分子 細胞骨架 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 83kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCDHB15. |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. Pcdhb22 (protocadherin beta 22), also known as PcdhbV or Pcdhb15, is a 794 amino acid murine protein that is thought to function as a calcium-dependent cell-adhesion protein. Pcdhb22 is a homolog of human PCDHB15 (protocadherin beta 15), a 787 amino acid protein that is one of 16 proteins in the protocadherin beta cluster. Unlike the alpha and gamma gene clusters whose genes are spliced to downstream constant region exons during transcription, members of the beta cluster (such as PCDHB15) do not use constant-region exons to produce mRNAs. As a result, each protocadherin beta gene encodes the transmembrane, extracellular and short cytoplasmic domains of the protein. Function: Protocadherin beta 15 (PCDHB15) is a member of the protocadherin family. Protocadherins constitute the largest subgroup within the cadherin family of calcium-dependent cell-cell adhesion molecules. They are neural cell surface proteins which are present at synaptic junctions. Their specific functions are unknown but they may be involved in the establishment and function of specific neuronal connections in the brain. PCDHB15 is one of sixteen tandemly arranged genes in the PCDHB gene cluster on chromosome 5q31. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Similarity: Contains 6 cadherin domains. Database links: Entrez Gene: 56121 Human Omim: 606341 Human SwissProt: Q9Y5E8 Human Unigene: 130757 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
