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Rabbit Anti-Nidogen/Biotin Conjugated antibody (bs-6911R-Bio)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-6911R-Bio
英文名稱 Rabbit Anti-Nidogen/Biotin Conjugated antibody
中文名稱 生物素標記的巢蛋白1抗體
別    名 Ent; Entactin 1; Entactin; NID 1; NID; NID-1; Nid1; Nidogen (enactin); Nidogen 1; Nidogen; Nidogen-1; NID1_HUMAN.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  免疫學  神經(jīng)生物學  信號轉導  細胞周期蛋白  細胞粘附分子  細胞分化  細胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse, Rat,  (predicted: Human, Cow, Rabbit, )
產品應用 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 133kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Entactin
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is a highly conserved member of the Nidogen family, which also includes Nidogen-2. Nidogen-2 has a high level of N- and O-glycosylation, and it interacts with Collagens Type I and IV and Perlecan at a comparable level to Nidogen. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation. Nidogen also cooperates with Laminin 1 to regulate b-casein expression.

Function:
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Subunit:
Interacts with FBLN1 and LGALS3BP. Interacts with PLXDC1.

Subcellular Location:
Secreted, extracellular space, extracellular matrix, basement membrane.

Post-translational modifications:
N- and O-glycosylated.

Similarity:
Contains 6 EGF-like domains.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain.

Database links:

Entrez Gene: 534319 Cow

Entrez Gene: 4811 Human

Entrez Gene: 18073 Mouse

Entrez Gene: 25494 Rat

Omim: 131390 Human

SwissProt: P14543 Human

SwissProt: P10493 Mouse

SwissProt: P08460 Rat

Unigene: 356624 Human

Unigene: 4691 Mouse

Unigene: 105658 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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